Summary of Features

Additional Non-Mammalian Model Organisms
(Continued)
February 1999

 

G. gallus
(Chicken)

C. reinhardtii
(Chlamydomonas)

F. rubripes
(Fugu)

Genome

haploid chromosome #

38 (8 macro/30 micro)

17

 

Size

~1,200 Mb( 990Mb-210 Mb)

~100Mb

~400Mb

fraction sequenced

<1%

~2%

~10,000 scanned; 0.5% sequenced

genes/ORFs

80,000 to 100,000

~10,000

cDNA/EST sequence

 

 

 

     

Characteristics

     

cellular organization

complex multicellular

unicellular

complex

Ploidy

diploid

haploid (diploids can be constructed)

diploid

generation time

6 hrs to 8 hrs mitotic; 7 days full life cycle

not relevant

(egg->egg)

5 months

   

Cultivation

yes

simple, inexpensive

not relevant

 

 

 

 

Transfection/transgenic

Yes (retroviral)/ Yes (inefficiently)

yes

not relevant

gene inactivation

no

Transforming DNA insertion

 
     

Mutants

~250

~200 mapped genetically; ~150 unmapped well characterized; ~1000 alleles; ~200 molecular markers mapped

not relevant; 21,000 species of teleost fish

     

Cell Culture

yes

yes

irrelevant

     

Storage

     

gametes

semen, yes

   

embryos

no (blastodisc-yes; primordial germ cells-yes; ES cells-yes)

   

intact organisms

no

Intact organism frozen or as dried zygotes

irrelevant

       
       
 

G. gallus

C. reinhardtii

F. rubripes

       

Special Strengths

accessibility of embryo for experimental manipulation (microsurgery or retroviral delivery); availability; economical

unicellular; colonial relatives haploid; cloning by complementation feasible; analysis of flagellum/basal body and chloroplast

No repetitive DNA sequences; small introns; ~1 gene/6-7kb

     

Weaknesses

no ESTs; no consolidated databases

unicellular but large genome size; no reliable homologous recombination for nuclear genome

 
     

Resources

mutant stocks

stock center

(www.botany.duke.edu/chlamy/)

 
     

Databases

http://www.ri.bbsrc.ac.uk/chick map; http://poultry.mph.msu.edu/mapsnew.html; http://ag.ansc.purdue.edu/poultry/; http://www.zod.wau.nl/vf/chickensite/chicken.html; http://tetra.gig.usda.gov:8400/chickgbase/manager.html;

Report by Genetic Resource Conservation Program UC Davis (in press)

http://probe.nalusda.gov:8300/cgi-bin/browse/chlamydb

http://fugu.hgmp.mrc.ac.uk/

     

Community

     

# of labs

~200 (US)

~400

~5

# of investigators

~800

~1,200

~20

     

References

Methods in Avian Embryology (1996) M. Bronner-Fraser

Morgan, B.A. and Fekete, D.M. (1996), Manipulating Gene Expression with Replication-competent Retroviruses: Methods in Avian Embryology (1996)

M. Bronner-Fraser, Ed.

Burt, D.W., and Cheng, H.H. (1998) The Chicken Gene Map, ILAR J. 38:229-236.

Smith, J. and Burt, D.W. (1998) Parameters of the Chicken Genome (Gallus gallus) Animal Genetics 29: 290-294. ed.

Dutcher, S.K. (1995). Flagellar assembly in 250 easy-to-follow steps. Trends Genet. 11:398-404.

Harris, E.H. (1989). The Chlamydomonas Sourcebook. A Comprehensive Guide to Biology and Laboratory Use. San Diego: Academic Press.

Lefebvre, P.A., and C.D. Silflow (1999). Chlamydomonas: The cell and its genome. Genetics 151: 9-14.

Rochaix, J.-D., M. Goldschmidt-Clermont and S. Merchant, eds. (1998). Molecular Biology of Chlamydomonas: Chloroplasts and Mitochondria. Dordrecht: Kluwer Academic Publishers.

See MEDLINE Fugu

     

Information Provided By:

P. Goetinck

U. Goodenough; S. Dutcher;

P. Lefevbre; E. Harris

S. Brenner

5/10/99 1:28 PM      

<< Back

Table of Content

Next >>