Rat BAC End Sequencing Project
The Institute for Genomic Research (TIGR) has been funded by the
National Institutes of Health to generate sequences from both ends
of ~200,000 rat BAC clones from CHORI-230 library by July 2002.
The quality of the sequences meet the following criteria: 1) a two-ends
clone fraction of 80%; 2) an average read length of 550 bp and an
average Q20 length of 470 bp; and 3) a clone tracking accuracy of
at least 98%. As on October 2001, with a sequencing success rate
of 80%, we have produced ~80,000 end sequences from ~44,500 CHORI-230
EcoRI segment clones with 77% of the clones having paired-ends.
We will collect any BAC end sequences that are publicly available
from any sites and are annotating each sequence for repeats, ESTs,
STSs and finished genomic sequences. The available BAC map data
will be gathered from various genome centers.
The rat BAC ends database at TIGR is daily updated to integrate
the BAC end sequences, the phred quality scores, the map, and the
annotation data for each BAC clone. The database provides links
to sites of the library information, the reports of GenBank and
dbGSS, and other relevant data. The database is freely accessible
via the web and supports sequence or clone searches and anonymous
FTP. The relevant sites and resources are described at http://www.tigr.org/tdb/bac_ends/rat/bac_end_intro.html.
Additional information about the database can be obtained from:
Shaying Zhao, Ph.D.
The Institute for Genomic Research
9712 Medical Center Drive
Rockville, MD 20850