Rat BAC End Sequencing Project

The Institute for Genomic Research (TIGR) has been funded by the National Institutes of Health to generate sequences from both ends of ~200,000 rat BAC clones from CHORI-230 library by July 2002. The quality of the sequences meet the following criteria: 1) a two-ends clone fraction of 80%; 2) an average read length of 550 bp and an average Q20 length of 470 bp; and 3) a clone tracking accuracy of at least 98%. As on October 2001, with a sequencing success rate of 80%, we have produced ~80,000 end sequences from ~44,500 CHORI-230 EcoRI segment clones with 77% of the clones having paired-ends. We will collect any BAC end sequences that are publicly available from any sites and are annotating each sequence for repeats, ESTs, STSs and finished genomic sequences. The available BAC map data will be gathered from various genome centers.

The rat BAC ends database at TIGR is daily updated to integrate the BAC end sequences, the phred quality scores, the map, and the annotation data for each BAC clone. The database provides links to sites of the library information, the reports of GenBank and dbGSS, and other relevant data. The database is freely accessible via the web and supports sequence or clone searches and anonymous FTP. The relevant sites and resources are described at http://www.tigr.org/tdb/bac_ends/rat/bac_end_intro.html.

Additional information about the database can be obtained from:
Shaying Zhao, Ph.D.
Assistant Investigator
The Institute for Genomic Research
9712 Medical Center Drive
Rockville, MD 20850
E-mail: szhao@tigr.org
TEL: 301-838-3532
FAX: 301-838-0208

 

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