* Senior Investigator: B.K. Lee

* Affiliation: Laboratory of Molecular Biology, NCI.

* Research:

* Characterization of the forces that govern protein stability and its interaction with ligands and other macromolecules.

* Protein structure prediction by Monte Carlo calculation and by pattern recognition.

* Design of mutations on a class of immunotoxins that will improve their property and biological activity.

* Design and development of molecular graphics and other software tools for molecular modeling.

* Personnel/Resources: Joe Cammisa (system manager); Bhupinder Madan (hydrophobicity); Natalya Kurochkina (protein folding by Monte Carlo); Sun Hee Jung (immunotoxin engineering); Yuri Sergeev (protein structure prediction by pattern recognition).

There is one personal IRIS or Indigo SGI graphics workstation for each member of the group and a couple more for visitors and summer students. In addition, the group has two IBM RISC 6000/560's and an SGI 4D340 4-processor machine. The group has easy access to Convex and Intel computers of the DCRT. The Cray computer of NCI at Frederick is also available.

* Publications:

H.S. Kang, N.A. Kurochkina, & B. Lee (1993). J. Mol. Biol. 229:448-460. Estimation and use of protein backbone angle probabilities.

B. Lee (1993). Protein Science 2:733-738. Estimation of the maximum change in stability of globular proteins upon mutation of a hydrophobic residue to another of smaller size.

B. Lee (1991). Proc. Natl. Acad. Sci. USA 88:5154-5158. Isoenthalpic and isoentropic temperatures and the thermodynamics of protein denaturation.

* Contact: Dr. B.K. Lee, Bldg. 37, Room 4B15. NCI, NIH, Bethesda, MD 20892.

Tel: (310) 496-6580 Fax: (301) 402-1344

E-mail: BKL@HELIX.NIH.GOV.

Figure: Engineered disulfide bridge between the heavy and light chains of mouse monoclonal antibody B3.