* Senior investigator: Ad Bax

* Affiliation: Laboratory of Chemical Physics, NIDDK.

* Research: Development and application of improved NMR methods for the study of macromolecular structure and dynamics.

* Development of methods that provide additional structural constraints for obtaining higher definition of macromolecular structure.

* Development of methods and procedures for facilitating the resonance assignment process.

* Development of methods for a better characterization of internal and overall macromolecular dynamics.

* Development of methods for extending the molecular weight limit of systems that can be studied.

* Personnel/Resources: Stephan Grzesiek, Geerten Vuister, Frank Delaglio, Andy Wang, Guang Zhu.

The group is equipped with a 600 MHz Bruker AMX-600 NMR spectrometer capable of triple resonance and pulsed field gradient experiments, and shares 500 MHz spectrometers with the groups of G.M. Clore, A.M. Gronenborn and D.A. Torchia.

* Publications:

A. Bax, & S. Grzesiek (1993). Acc. Chem. Res. 26:131-138. Methodological advances in protein NMR.

A. Bax, D. Max, & D. Zax (1992). J. Am. Chem. Soc. 114:6923-6925. Measurement of multiple-bond 13C-3C J couplings in a 20-kDa protein-peptide complex.

S. Grzesiek, H. Doebeli, R. Gentz, G. Garotta, A.M. Labhardt, & A. Bax (1992). Biochemistry 31:8180-8190. 1H, 13C, and 15N NMR backbone assignments and secondary structure of human interferon-[[gamma]].

L.E. Kay, G. M. Clore, A. Bax, & A.M. Gronenborn (1990). Science 249:411-414. Four-dimensional heteronuclear triple-resonance NMR spectroscopy of interleukin-1ß in solution.

G. Barbato, M. Ikura, L.E. Kay, & A. Bax (1990). Biochemistry 31:5269-5278. Backbone dynamics of calmodulin studied by 15N relaxation using inverse detected two-dimensional NMR spectroscopy: the central helix is flexible.

* Contact: Ad Bax, Bldg. 5, Room 126. NIDDK, NIH, Bethesda, MD 20892.

Tel: (301) 496-2848 Fax: (301) 402-0907