Ulrich Siebenlist
Chief, Immune Activation Section
National Institutes of Health
Bldg.10, Rm. 11B16
Bethesda, Maryland 20892-1876
Phone: (301) 496-7662
Fax: (301) 402-0070
Email: US3n@nih.gov
Research Interests
Current Main Research Topics of the Laboratory:
Molecular Immunology, Transcription Factors and Signaling:
A) Immune activation mechanisms, especially the study of activation-responsive genes of human blood-derived T cells.
B) Mechanisms of activation of the NF-kB transcription factors, including identification of components of intracellular signaling cascades leading to proteolytic degradation of inhibitors of NF-kB (e.g. MAP Kinase-like and TNF-response signaling pathways).
C) Role of NF-kB factors in regulating genes and in specific immune responses to pathogens by analysis of "knock-out" mice and transgenic mice. Role of NF-kB factors in tumorigenesis.
Recent Publications
- Baldi, L., Brown, K., Franzoso, G., Siebenlist, U. (1996). Critical role for lysines 21 and 22 in signal-induced, ubiquitin-mediated proteolysis of IkB-a. J. Biol. Chem., 271:376-379.
- Brown, K., Gerstberger, S., Carlson, L., Franzoso, G., Siebenlist, U. (1995). Control of IkB-a proteolysis by site-specific, signal-induced phosphorylation. Science 267:1485-1488.
- Kelly, K., Siebenlist, U. (1995). Immediate early genes induced by antigen receptor stimulation. Curr. Opin. in Immun., 7:327-332.
- Kanno, T., Brown, K., Siebenlist, U. (1995). Evidence in support of a role for HTLV-I Tax in activating NF-kB via stimulation of signaling pathways. J. Biol. Chem., 270:11745-11748.
- Lin, Y.-C., Brown, K., Siebenlist, U. (1995). Activation of NF-kB requires proteolysis of the inhibitor IkB-a: Signal-induced phosphorylation of IkB-a alone does not release active NF-kB. Proc. Natl. Acad. Sci. USA 92:552-556.
- Siebenlist, U., Brown, K., Franzoso, G. (1994). NF-kB: a mediator of pathogen and stress responses. In: Inducible Gene Expression, Vol. 1 (ed. P. Baeuerle) Birkhaeuser,Boston, pp.93-141.
- Kanno, T., Franzoso, G., Siebenlist, U. (1994). Human T-cell leukemia virus type I Tax-protein-mediated activation of NF-kB from p100 (NF-kB2)-inhibited cytoplasmic reservoirs. Proc. Natl. Acad. Sci. USA 91:12634-12638.
- Franzoso, G., Biswas, P., Poli, G., Carlson, LM, Brown K., Tomita-Yamaguchi, M., Fauci, AS, Siebenlist, U. (1994). A family of serine proteases expressed exclusively in myelo-monocytic cells specifically processes the NF-kB subunit p65 in vitro and may impair HIV replication in these cells. J. Exp. Med. 180:1445-1456.
- Kanno, T., Brown, K., Franzoso, G., Siebenlist, U. (1994). Kinetic analysis of HTLV-I Tax-mediated activation of NF-kB. Mol. Cell. Biol. 14:6443-6451.
- Siebenlist, U., Franzoso, G., Brown, K. (1994). Structure, function and regulation of NF-kB. Annu. Rev. Cell Biol. 10:405-455.
- Lenardo, M., Siebenlist, U. (1994). Bcl-3-mediated nuclear regulation of the NF-kB trans-activating factor. Immunol. Today 15:145-146.
- Maguire, J., Santoro, T., Jensen, P., Siebenlist, U., Yewdell, J., Kelly, K. (1994). Gem: an induced, immediate-early protein belonging to the Ras family. Science 265:241-244.
- Franzoso, G., Bours, V., Azarenko, V., Park S., Tomita-Yamaguchi, M., Kanno, T., Brown, K., Siebenlist, U. (1993). The oncoprotein Bcl-3 can facilitate NF-kB-mediated transactivation by removing inhibiting p50 homodimers from select B sites. EMBO J.12:3893-3901.
- Bours, V., Azarenko, V., Dejardin, E., Siebenlist, U. (1993). The human RelB (I-Rel) functions as a kB site-dependent transactivating member of the family of Rel-related proteins. Oncogene 9:1699-1702.
- Rohan, P., Davis, P., Moskaluk, C., Kearns, M., Krutzsch, H., Siebenlist, U. and Kelly, K. (1993). PAC-1: A mitogen-induced nuclear tyrosine phosphatase, Science 259:1763-1766.
- Bours, V., Franzoso, G., Azarenko, V., Park, S., Kanno, T., Brown, K., Siebenlist, U. (1993). The Oncoprotein Bcl-3 directly transactivates through kB motifs via association with DNA-Binding p50B homodimers. Cell 12:729-739.
- Brown, K., Park, S., Kanno, T., Franzoso, G., and Siebenlist, U. (1993). Mutual regulation of the transcriptional activator NF-kB and its inhibitor, IkB-a. Proc. Natl. Acad. Sci. U.S.A. 90:2532-2536.
- Bressler, P., Brown, K., Timmer, W., Bours, V., Siebenlist, U. and Fauci, A.S. (1993). Mutational analysis of the NF-kB p50 protein and inhibition of NF-kB activity by trans-dominant mutants. J. Virol. 67:288-293.
- Franzoso, G., Bours, V., Park, S., Tomita-Yamaguchi, M., Kelly, K. and Siebenlist, U. (1992). The candidate oncoprotein Bcl-3: an antagonist of p50/NF-kB-mediated inhibition. Nature 359:339-342.
- Bours, V., Franzoso, G., Brown, K., Park, S., Azarenko, V., Tomita-Yamaguchi, M., Kelly, K. and Siebenlist, U. (1992). Lymphocyte activation and the family of NF-kB transcription factor complexes. In: Current Topics in Microbiology and Immunology, Eds. Springer Verlag, Berlin-Heidelberg 182:411-420.
- Zipfel, P., Wharton, K., Villalobos, J., Irving, S., Kelly, K. and Siebenlist, U. (1992). Employment of antipeptide antisera to distinguish two closely related cytokines induced in human T cells. Lymphokine and Cytokine Research 11:141-148.
- Kelly, K., Davis, P., Mitsuya, H., Irving, S., Wright, J., Grassmann, R., Fleckenstein, B., Wano, Y., Greene, W. and Siebenlist, U. (1992). A high proportion of early response genes are constitutively activated in T cells by HTLV I. Oncogene 7:1463-1470.
- Ryseck, R.P., Bull, P., Takamiya, M., Bours, V., Siebenlist, U., Dobrazanski and Bravo, R. (1992). RelB, a new Rel family transcription activator that interacts with p50-NF-kB. Mol. Cell. Biol. 12:674-684.
- Bours, V., Burd, P.R., Brown, K., Villalobos, J., Park, S., Ryseck, R.P., Bravo, R., Kelly, K. and Siebenlist (1992). A novel mitogen-inducible gene product related to p50/p105/NF-kB participates in transactivation through a kB site. Mol. Cell. Biol. 12:685-695.
- Bours, V., Villalobos, J., Burd, P.R., Kelly, K. and Siebenlist, U.(1990). Cloning of a mitogen-inducible gene encoding a kB DNA-binding protein with homology to the rel oncogene and to cell cycle motifs. Nature 348:76-80.
- Schmidt, A., Hennighausen, L. and Siebenlist, U. (1990). Inducible nuclear factor binding to the "Kappa" B elements of the human immunodeficiency virus enhancer in T cells can be blocked by cyclosporin A in a signal-dependent manner. J. Virol.64:4037-4041.
- Wright, J., Gunter, K.C., Mitsuya, H., Irving, S.G., Kelly, K. and Siebenlist, U. (1990). Expression of a zinc finger gene in HTLV-I- and HTLV-II- transformed cells. Science 248:588-591.
- Irving, S.G., Zipfel, P.F., Balke, J., McBride, O.W., Morton, C.C., Burd, P.R., Siebenlist, U. and Kelly, K. (1990). Two inflammatory mediator cytokine genes are closely linked and variably amplified on chromosome 17q. Nucleic Acids Res. 18:3262-3269.
- Gunter, K.C., Irving, S.G., Zipfel, P.F., Siebenlist, U. and Kelly, K (1989). Cyclosporin A-mediated inhibition of mitogen-induced gene transcription is specific for the mitogenic stimulus and cell type. J. Immunol. 142:3286-3291.
- Zipfel, P.F., Balke, J. Irving, S.G., Kelly, K. and Siebenlist, U.(1989). Mitogenic activation of human T cells induces two closely related genes which share structural similarities with a new family of secreted factors. J. Immunol. 142:1582-1590.
- Irving, S.G., June, C.H., Zipfel, P.F., Siebenlist, U. and Kelly, K. (1989). Mitogen induced genes are subject to multiple pathways of regulation in the initial stages of T cell activation. Molec. Cellular Biol. 9:1034-1040.
- Zipfel, P.F., Irving, S.G., Kelly, K. and Siebenlist. U. (1989). Complexity of the primary genetic response to mitogenic activation of human T cells. Molec. Cellular Biol. 9:1041-1048.
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Last modified on February 9, 1996.